Journal: Journal of Nanobiotechnology

Article Title: SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

doi: 10.1186/s12951-022-01446-1

Figure Lengend Snippet: miR-1246 as a SHED-Exo-enriched miRNA that stimulating macrophage autophagy. a The miRNA expression levels of SHED-derived exosomes were determined by qRT-PCR. Relative quantification was performed using cel-miR-39-3p for normalization. b THP-1 cells were incubated with 20 μg/mL SHED-Exo or transfected with 50 nM of miRNA mimics as indicated. The miR-1246, miR-100-5p, and miR-92a-3p levels were determined by qRT-PCR. Relative quantification was performed using U6 small nuclear RNA for normalization. c Gene expression levels of autophagy markers Lc3b in THP-1 macrophages per group. d THP-1 cells were transfected with 50 nM of miR-1246 mimic and 100 nM of miR-1246 inhibitor. Western blot results and relative expression levels of LC3, AKT, ERK1/2, and STAT3 signaling pathway proteins per treatment group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

Article Snippet: Mouse skin samples and THP-1 cells were homogenized using TRIzol reagent (Ultrapure RNA Kit, CW Biotech).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Incubation, Transfection, Western Blot